APM was administered to rats given BHP (t-butylhydroperoxide), one of radical-producing compounds to determine antioxidation activity of APM in the liver. As a result, it was shown that APM had no less antioxidative activity than vitamin C.

■Antioxidation activity of APM.

It has also been demonstrated in chickens that APM exhibits radical trapping activity against peroxy radicals and alkoxy radicals.

■Radical trapping activityof APM.

Effect of L-Ascorbic Acid-2- Phosphate Magnesium on Chicks Administered a Radical Initiator

By Yosuke AOYAGi, Katsuhide NARUMIYA, Shinobu ITO

REFERENCE:Jpn. Pauli. Sci., 33 : 383-38i 1996

The present study was conducted to investigate the effects of 2, 2'-azobis (amidinopropane) dihydrochloride (AAPH), a radical initiator, and L-ascorbic acid-2-phosphate magnesium (APM) on growth performance and ascorbic acid concentrations in Single Comb White Leghorn male chicks. Body weight gain, food intake and food effciency (body weight gain/Oood intake) of chicks administered with AAPH (5 mglioog body weight/day) for 14 days were significantly decreased compared with control values. However, the supplementation with APM reduced the severity of such adverse effects induced by the administration of AAPH. In chicks administered with AAPH, plasma ascorbic acid wassignificantly depressed, and the concentrations of plasma and liver thiobarbituric acid reactive substances (TBARS) were significantly increased. The increased TBARS concentrations in plasma and liver were restored by dietary APM supplementation.

Based on the results presented, it was suggested that the chicks given AAPH could serve as an model animal of radical injury, and that APM supplementation is effective for inhibition of oxidative stress by peroxyl radical generated from AAPH.

Effect of Heat Stress and L-Ascorbic Acid-2-Phosphate Magnesium on Plasma and Liver Thiobarbituric Acid Reactive Substances Concentrations,and on Liver Protein Carbonyl Concentration in Chicks

By Yosuke AOYAGI, Takashi OHNISHI, Shinobu ITO and Tetsuro NAKAYA

REFERENCE:Jan. Pault. Sci., 34 : 63-66, 1997

The present study was conducted to investigate the effect o. fheat stress and dietary L- ascorbic acid-2-phosphate magnesium (APM) on chicks at the age of 7 days old. The plasma and liver concentrations of thiobarbituric acid reactive substances (TBARS) of chicks reared at 400C for 3 days were significantly increased compared with values of control chicks (30oc). The liver protein carbonyl level of chicks after heat exposure for 3 days tended'to be increased. By dietary APM, the increased liver TBARS concentration was significantly decreased, and the concentrations of plasma TBARS and liver protein carbonyl tended to be decreased.

Based on the results presented, it was suggested that heat exposure causes oxidative stress in chicks, and that the administration of APM is effective for inhibition of oxidative stress by heat exposure.